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KMID : 0355820210420010027
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Korean Journal of Oral Anatomy
2021 Volume.42 No. 1 p.27 ~ p.35
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Cimicifuga rhizome extract induces apoptosis through caspase-3 activation in SCC 25 cells
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Choi Byul-Bo-Ra
Park Sang-Rye
Kim Gyoo-Cheon
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Abstract
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This study was undertaken to verify the induction and pathway of apoptosis in oral squamous carcinoma cells (SCC 25) using cimicifuga rhizome extract. In the present study, the WST-1 assay was used to detect the cell viability of cimicifuga rhizome extract on SCC 25 cells. Immuno-fluorescence and flow cytometry assays were used to detect cell apoptosis. The effect of cimicifuga rhizome extract on apoptosis-associated proteins (PARP-1, caspase-3 and caspase-9) were determined by western blot. We found that cimicifuga rhizome extract significantly inhibited cell viability in a dose-dependent manner in SCC 25 cells. Also, cimicifuga rhizome extract increased the sub G1 and changed the cytochrome c and AIF location. In addition, we observed that the caspase-3 signaling pathway played a key role in cimicifuga rhizome extract induced apoptosis. The results of the present study showed that cimicifuga rhizome extract promotes apoptosis via regulating the caspase-3 signaling pathway in SCC 25 cells.
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KEYWORD
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apoptosis, cimicifuga rhizome, oral squamous cell carcinoma
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